Journal: Antimicrobial Agents and Chemotherapy

Article Title: Urinary Tract Physiological Conditions Promote Ciprofloxacin Resistance in Low-Level-Quinolone-Resistant Escherichia coli

doi: 10.1128/AAC.00602-16

Figure Lengend Snippet: Genotypes of isogenic and UPEC strains a

Article Snippet: These results indicate that anaerobic growth in urine increased the CIP MIC at pH 7 (2-fold to 8-fold), pH 6 (16-fold to 64-fold), and pH 5 (128-fold to 512-fold). table ft1 table-wrap mode="anchored" t5 TABLE 4 caption a7 Strain MIC (μg/ml) in MH broth and urine at pH: 7 6 5 O 2 nO 2 O 2 nO 2 O 2 nO 2 ATCC 25922 4 8 32 64 128 256 EC01 1 8 8 64 32 256 EC02 4 8 16 32 128 256 EC03 2 8 16 64 32 128 EC04 2 4 16 32 64 128 EC05 1 4 16 64 64 256 EC06 4 8 16 32 128 256 EC07 2 8 16 64 64 256 EC08 2 4 16 32 128 256 EC09 1 4 16 64 32 128 Open in a separate window a O 2 , aerobic incubation; nO 2 , anaerobic incubation.

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Urinary Tract Physiological Conditions Promote Ciprofloxacin Resistance in Low-Level-Quinolone-Resistant Escherichia coli

doi: 10.1128/AAC.00602-16

Figure Lengend Snippet: Ciprofloxacin MICs for E. coli strains in urine at different pH values a

Article Snippet: These results indicate that anaerobic growth in urine increased the CIP MIC at pH 7 (2-fold to 8-fold), pH 6 (16-fold to 64-fold), and pH 5 (128-fold to 512-fold). table ft1 table-wrap mode="anchored" t5 TABLE 4 caption a7 Strain MIC (μg/ml) in MH broth and urine at pH: 7 6 5 O 2 nO 2 O 2 nO 2 O 2 nO 2 ATCC 25922 4 8 32 64 128 256 EC01 1 8 8 64 32 256 EC02 4 8 16 32 128 256 EC03 2 8 16 64 32 128 EC04 2 4 16 32 64 128 EC05 1 4 16 64 64 256 EC06 4 8 16 32 128 256 EC07 2 8 16 64 64 256 EC08 2 4 16 32 128 256 EC09 1 4 16 64 32 128 Open in a separate window a O 2 , aerobic incubation; nO 2 , anaerobic incubation.

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Urinary Tract Physiological Conditions Promote Ciprofloxacin Resistance in Low-Level-Quinolone-Resistant Escherichia coli

doi: 10.1128/AAC.00602-16

Figure Lengend Snippet: MICs of ciprofloxacin for isogenic E. coli strains in MH broth at different pH values a

Article Snippet: These results indicate that anaerobic growth in urine increased the CIP MIC at pH 7 (2-fold to 8-fold), pH 6 (16-fold to 64-fold), and pH 5 (128-fold to 512-fold). table ft1 table-wrap mode="anchored" t5 TABLE 4 caption a7 Strain MIC (μg/ml) in MH broth and urine at pH: 7 6 5 O 2 nO 2 O 2 nO 2 O 2 nO 2 ATCC 25922 4 8 32 64 128 256 EC01 1 8 8 64 32 256 EC02 4 8 16 32 128 256 EC03 2 8 16 64 32 128 EC04 2 4 16 32 64 128 EC05 1 4 16 64 64 256 EC06 4 8 16 32 128 256 EC07 2 8 16 64 64 256 EC08 2 4 16 32 128 256 EC09 1 4 16 64 32 128 Open in a separate window a O 2 , aerobic incubation; nO 2 , anaerobic incubation.

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Urinary Tract Physiological Conditions Promote Ciprofloxacin Resistance in Low-Level-Quinolone-Resistant Escherichia coli

doi: 10.1128/AAC.00602-16

Figure Lengend Snippet: Mean fold changes in MICs of ciprofloxacin in MH broth and urine at different values of pH and anaerobiosis a

Article Snippet: These results indicate that anaerobic growth in urine increased the CIP MIC at pH 7 (2-fold to 8-fold), pH 6 (16-fold to 64-fold), and pH 5 (128-fold to 512-fold). table ft1 table-wrap mode="anchored" t5 TABLE 4 caption a7 Strain MIC (μg/ml) in MH broth and urine at pH: 7 6 5 O 2 nO 2 O 2 nO 2 O 2 nO 2 ATCC 25922 4 8 32 64 128 256 EC01 1 8 8 64 32 256 EC02 4 8 16 32 128 256 EC03 2 8 16 64 32 128 EC04 2 4 16 32 64 128 EC05 1 4 16 64 64 256 EC06 4 8 16 32 128 256 EC07 2 8 16 64 64 256 EC08 2 4 16 32 128 256 EC09 1 4 16 64 32 128 Open in a separate window a O 2 , aerobic incubation; nO 2 , anaerobic incubation.

Techniques:

Journal: Scientific Reports

Article Title: Rapid bacterial antibiotic susceptibility test based on simple surface-enhanced Raman spectroscopic biomarkers

doi: 10.1038/srep23375

Figure Lengend Snippet: ( a ) E. coli (ATCC 25922); ( b ) clinical isolate of imipenem-resistant E. coli with (red curves) and without (black curves) imipenem treatment; ( c ) signal ratio of 654-cm −1 SERS peak ( r 654 ) of E. coli (ATCC 25922) as a function of imipenem treatment time; ( d ) r 654 of imipenem-resistant E. coli as a function of imipenem treatment time; ( e ) signal ratio of 724-cm −1 SERS peak ( r 724 ) of E. coli (ATCC 25922) as a function of imipenem treatment time; ( f ) r 724 of imipenem-resistant E. coli as a function of imipenem treatment time. Black and red curves represent the mean SERS spectra, while gray and light red curves represent their corresponding standard deviation.

Article Snippet: Methicillin-susceptible Staphylococcus aureus (ATCC 29213) and Escherichia coli (ATCC 25922 and ATCC 35218) were obtained from American Type Culture Collection (ATCC).

Techniques: Standard Deviation

Journal: Scientific Reports

Article Title: Rapid bacterial antibiotic susceptibility test based on simple surface-enhanced Raman spectroscopic biomarkers

doi: 10.1038/srep23375

Figure Lengend Snippet: After being treated by antibiotics for 0, 0.5, 1 and 2 hours, the bacteria were stained with PI (fluorescing red) and SYTO 9 (fluorescing green). BF: bright-field image; FL: fluorescence image. ( a ) S. aureus (ATCC 29213) treated with oxacillin; ( b ) E. coli (ATCC 25922) treated with imipenem; ( c ) percentage of dead bacteria ( f dead ): S. aureus (black columns) and E. coli (gray columns). f dead = N r /( N g + N r − N g + r ), where N r is the count of the red signatures, N g is the count of the green signatures, and N g + r is the count that the green and red signatures overlap. The scale bars represent 20 μ m.

Article Snippet: Methicillin-susceptible Staphylococcus aureus (ATCC 29213) and Escherichia coli (ATCC 25922 and ATCC 35218) were obtained from American Type Culture Collection (ATCC).

Techniques: Bacteria, Staining, Fluorescence

Journal: Scientific Reports

Article Title: Rapid bacterial antibiotic susceptibility test based on simple surface-enhanced Raman spectroscopic biomarkers

doi: 10.1038/srep23375

Figure Lengend Snippet: ( a ) Signal ratio of 730-cm −1 SERS peak ( r 730 ) of S. aureus versus vancomycin treatment time at concentrations of 0.5 μ g/ml (light gray columns), 1 μ g/ml (gray columns), and 2 μ g/ml (black columns); ( b , c ) signal ratios of 654-cm −1 SERS peak ( r 654 ) and 724-cm −1 SERS peak ( r 724 ) of E. coli , respectively, versus imipenem treatment time at concentrations of 0.03 μ g/ml (light gray columns), 0.06 μ g/ml (gray columns), and 0.12 μ g/ml (black columns).

Article Snippet: Methicillin-susceptible Staphylococcus aureus (ATCC 29213) and Escherichia coli (ATCC 25922 and ATCC 35218) were obtained from American Type Culture Collection (ATCC).

Techniques:

Journal: Scientific Reports

Article Title: Rapid bacterial antibiotic susceptibility test based on simple surface-enhanced Raman spectroscopic biomarkers

doi: 10.1038/srep23375

Figure Lengend Snippet: ( a – c ) Signal ratio of 654-cm −1 SERS peak ( r 654 ) of E. coli of inoculum densities of 10 6 , 10 7 and 10 8 CFU/ml, respectively, after being treated with different concentrations of imipenem for 2 hrs.

Article Snippet: Methicillin-susceptible Staphylococcus aureus (ATCC 29213) and Escherichia coli (ATCC 25922 and ATCC 35218) were obtained from American Type Culture Collection (ATCC).

Techniques: